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Genome diversification of P. putida KT2440. (A) P. putida KT2440/pSEVA428‐ACBE3 was subjected to ten cycles of mutagenesis. After plating, five single colonies were isolated and whole genome <t>sequencing</t> was performed. SNPs analysis for the genome and the plasmid is shown in the table, including small indels. For plasmid mutations, the gene/sequence affected, the SNP and the amino acid change are depicted. (B) Distribution of detected SNPs and indels (red bars) along the genome (blue line) is depicted for the five clones analysed.
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Genome diversification of P. putida KT2440. (A) P. putida KT2440/pSEVA428‐ACBE3 was subjected to ten cycles of mutagenesis. After plating, five single colonies were isolated and whole genome sequencing was performed. SNPs analysis for the genome and the plasmid is shown in the table, including small indels. For plasmid mutations, the gene/sequence affected, the SNP and the amino acid change are depicted. (B) Distribution of detected SNPs and indels (red bars) along the genome (blue line) is depicted for the five clones analysed.

Journal: Microbial Biotechnology

Article Title: Accelerated Adaptive Evolution of Gram‐Negative Bacteria With a Broad‐Host‐Range Dual Base Editor

doi: 10.1111/1751-7915.70450

Figure Lengend Snippet: Genome diversification of P. putida KT2440. (A) P. putida KT2440/pSEVA428‐ACBE3 was subjected to ten cycles of mutagenesis. After plating, five single colonies were isolated and whole genome sequencing was performed. SNPs analysis for the genome and the plasmid is shown in the table, including small indels. For plasmid mutations, the gene/sequence affected, the SNP and the amino acid change are depicted. (B) Distribution of detected SNPs and indels (red bars) along the genome (blue line) is depicted for the five clones analysed.

Article Snippet: Genome sequencing was outsourced to PlasmidSaurus, and SNPs were analysed using the company on‐line application.

Techniques: Mutagenesis, Isolation, Whole-Genome Sequencing, Analysis, Plasmid Preparation, Sequencing, Clone Assay